Slide preparation

Method

Material

• Silicon molds.
• Epoxy resin.
• Balance.
  Disposable pipette.
• Agitator.
• Plastic disposable Beaker.
• Acetone.
• Wiping paper
• Plastic tweezers
• Histology slides

 

Technic

1. Prepare epoxy resin by mixing the componant under an extractor fan, respect the proportions and measure with a precision libra.

2. Mix well for 5 min and leave it for 10 other min to release the bubbles.

3. Place each right otolith on the bottom of a mold cavity, external side face up.

4. Keep the reference of each otolith in each cavity.

5. Add drops of resin until the mold is filled up.

6. Remove the bubbles by gently moving the otolith.

7. Leave until the resin is hard.

8. Reference histology slides with the reference of the samples .

9. Remove the resin blocks with the embedded otoliths from the molds.

10. Melt cristal bound glue on a heating plate at 150° Celsius.

11. Put some liquid "Cristal bound" on the center of a referenced slide and quickly press the corresponding resin block upside down (the number facing upwards) on it.